Strains, culture conditions and chemicals Escherichia coli strain DH5α was used for plasmid construction. E. coli was grown at 37 °C and 300 r.p.m. in lysogeny broth (LB) liquid medium and at 37 °C on plates of LB solid medium supplemented with 20 g l–1 agar. Ampicillin was supplemented at a concentration of 100 mg l–1 for plasmid selection. The Y. lipolytica W29 strain (MATa, Y-63746 ARS Culture Collection, …
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