Tag Archives: yeast

Strain culture and DNA extraction We used a collection of S. cerevisiae isolates that were previously sequenced using short-read sequencing19. For each isolate, we obtained single colonies from frozen stock on solid YPD (1% yeast extract, 2% peptone, and 2% glucose) and cultured one colony per strain in 25 ml of liquid YPD at 30 °C under shaking (120 rpm). After the culture …

Bacterial and fungal culture conditions Fungal strains, bacterial strains and plasmids used in this study are listed in Supplementary Tables 14 and 15. S. Typhimurium strains were grown at 37 °C in lysogeny broth (LB) medium (per litre: 10 g tryptone, 5 g yeast extract and 10 g sodium chloride). C. albicans strains were grown at 37 °C in YPD media (per litre: 10 g yeast …

Bees feeding on a synthetic pollen substitute – credit, Caroline Wood, Oxford U news A study team from Oxford University has identified a fermentation method that creates the perfect balanced diet for honey bees who can’t get enough natural pollen. Synthetic pollen substitutes are often fed to bees as a dietary supplement to natural pollen, but until now it’s been …

Strains, culture conditions and chemicals Escherichia coli strain DH5α was used for plasmid construction. E. coli was grown at 37 °C and 300 r.p.m. in lysogeny broth (LB) liquid medium and at 37 °C on plates of LB solid medium supplemented with 20 g l–1 agar. Ampicillin was supplemented at a concentration of 100 mg l–1 for plasmid selection. The Y. lipolytica W29 strain (MATa, Y-63746 ARS Culture Collection, …