Tissue slide preparation Mouse C57 embryo sagittal frozen sections (MF-104-11-C57 and MF-104-13-C57) were purchased from Zyagen. Freshly collected E11 or E13 mouse embryos were snap frozen in optimal cutting temperature (O.C.T.) compounds and sectioned at 7–10 μm thickness. Tissue sections were collected on poly-l-lysine-coated glass slides (Electron Microscopy Sciences, 63478-AS). Juvenile mouse brain tissue (P21) was obtained from the C57BL/6 mice …
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