Cell culture and cell lines The following CRC cell lines were obtained from the American Type Culture Collection (ATCC): HCT116 (ATCC, CCL-247), DLD1 (ATCC, CCL-221), RKO (ATCC, CRL-2577), HCT8 (ATCC, CCL-244), NCI-H508 (ATCC, CCL-253), Colo205 (ATCC, CCL-222), SW620 (ATCC, CCL-227), SW48 (ATCC, CCL-231) and HCT15 (ATCC, CCL-225). The DiFi, GP5D and HT55 cell lines were provided by J. Engelman, and …
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