Formulation of lipid nanoparticles Lipid nanoparticles were formulated following the Onpattro formulation and using a model oligonucleotide, siHPRT, as a cargo. Details regarding LNP formulation, mixing, and downstream processing are provided in the materials and methods. Briefly, a four-component lipid mixture was solubilized in ethanol to a total lipid concentration of 12.5 mM (MC3: DMG-PEG-2000: DSPC: cholesterol at a molar ratio …
Read More »Buffer optimization of siRNA-lipid nanoparticles mitigates lipid oxidation and RNA-lipid adduct formation
