Cell strains The E. coli K-12 MG1655 strain was grown in Luria–Bertani medium at 37 °C with shaking at 200 rpm until reaching an OD600 of approximately 0.6. For heat shock treatment, cells were incubated in a water bath at 44 °C for 10 min. For transcription inhibition, cells were treated with 25 or 750 µg ml−1 Rif for 40 min at 37 °C and 200 rpm. To induce …
Read More »Elementary 3D organization of active and silenced E. coli genome
