Tag Archives: Signalling

Bacterial strains and growth conditions Standard bacterial cultures were grown in lysogeny broth (LB Lennox) at 37 °C and 250 rpm except where stated otherwise. When necessary, 34 µg ml−1 chloramphenicol (+Ch), +50 µg ml−1 kanamycin sulfate (+K) or +50 µg ml−1 carbenicillin (+C) was supplemented to media and LB agar plates. For cellular assays, all bacterial strains were stored at −80 °C in 25% glycerol (Sigma) when not …

Design of structured switch–binder fusions (hosts) allosterically coupling the target and the effector In PyMOL, we manually positioned the switch relative to the binder–target complex subject to several constraints: there is no steric overlap between the target and switch state X; there is large steric overlap between the target and switch state Y; the smallest deformation that could be undergone …

Animals Mice (8–40 weeks old) were housed under controlled light conditions (12 h light/12 h dark) and temperature (22 °C) and fed ad libitum on standard mouse chow. Mice were randomized to treatment group on the basis of body weight. Unless noted otherwise, all mice were male. Mice were group housed except for studies for food intake measurements. Mice used were: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J, with Cre-dependent tdTomato …

Key takeaways: XRP price just hit multiyear highs of $3.66, but technicals suggest there’s more room to run. Multiple XRP charts signal a potential “explosive” move, targeting $7-$20 if key resistances are broken. XRP (XRP) price has risen significantly over the last seven days, climbing to a new record high of $3.66 on Friday, and up nearly 90% since its …