Isolation of nuclei from post-mortem brain tissue Batch 1 nuclei (BA10 region, frozen tissue) were isolated according to a protocol adapted from a previous study18, performed entirely at 4 °C or on ice. In brief, tissue was homogenized (700 µl homogenization buffer: 320 mM sucrose, 5 mM CaCl2, 3 mM Mg(CH3COO)2, 10 mM Tris-HCl pH 7.8, 0.1 mM EDTA pH 8.0, 0.1% IGEPAL CA-630, 1 mM β-mercaptoethanol, 0.4 U µl−1 recombinant RNase …
Read More »ABCA7 variants impact phosphatidylcholine and mitochondria in neurons
