Tag Archives: phage

Isolation and characterization of phages against Enterobacter isolates from The Alfred Hospital The Alfred Hospital in Melbourne, Australia, has reported an ongoing outbreak of nosocomial infections with limited treatment options and high mortality rates29. Of particular concern is the ECC, which is an emerging AMR threat with considerable epidemic potential22. Here we explored phages as a frontline antimicrobial solution for …

ASO design ASOs were designed to bind at the RBS using the MASON algorithm (https://mason.helmholtz-hiri.de)34 and the NCBI sequence and annotation files (ΦKZ: NC_004629.1, ΦPA3: NC_028999.1, PP7: NC_001628.1, SPO1: NC_011421.1, RAY: NC_041973.1, PA14: NC_008463.1, PAO1: NC_002516.2). ASO length was set to 11 nt and the allowed mismatches for off-targets were set to 4 nt. ASOs were selected based on the following scoring values: …