Tag Archives: oxidation

Formulation of lipid nanoparticles Lipid nanoparticles were formulated following the Onpattro formulation and using a model oligonucleotide, siHPRT, as a cargo. Details regarding LNP formulation, mixing, and downstream processing are provided in the materials and methods. Briefly, a four-component lipid mixture was solubilized in ethanol to a total lipid concentration of 12.5 mM (MC3: DMG-PEG-2000: DSPC: cholesterol at a molar ratio …

Phylogenetic framework and HMMs of sulfur-cycling proteins To discern sulfur-cycling genes or proteins from their functionally divergent homologues, phylogenetic analysis was conducted for 116 sulfur-cycling proteins (Supplementary Table 1). For each protein family, sequences of enzymes with biochemically validated functions, including related sequences of enzymes with divergent functions (outgroups), were identified by literature surveys and recovered from SwissProt61. Additional homologues …