ASO design ASOs were designed to bind at the RBS using the MASON algorithm (https://mason.helmholtz-hiri.de)34 and the NCBI sequence and annotation files (ΦKZ: NC_004629.1, ΦPA3: NC_028999.1, PP7: NC_001628.1, SPO1: NC_011421.1, RAY: NC_041973.1, PA14: NC_008463.1, PAO1: NC_002516.2). ASO length was set to 11 nt and the allowed mismatches for off-targets were set to 4 nt. ASOs were selected based on the following scoring values: …
Read More »Programmable antisense oligomers for phage functional genomics
