Tag Archives: Neurons

Isolation of nuclei from post-mortem brain tissue Batch 1 nuclei (BA10 region, frozen tissue) were isolated according to a protocol adapted from a previous study18, performed entirely at 4 °C or on ice. In brief, tissue was homogenized (700 µl homogenization buffer: 320 mM sucrose, 5 mM CaCl2, 3 mM Mg(CH3COO)2, 10 mM Tris-HCl pH 7.8, 0.1 mM EDTA pH 8.0, 0.1% IGEPAL CA-630, 1 mM β-mercaptoethanol, 0.4 U µl−1 recombinant RNase …

Summary: Researchers studying people with epilepsy have discovered that nerve cells in the medial temporal lobe coordinate their firing with slow brain waves to encode and retrieve memories. This synchronization, known as theta-phase locking, occurs at one to ten cycles per second and is active during both learning and recall. The strength of this rhythm during memory formation did not …

Surprising Alzheimer’s breakthrough: Sugar in neurons might be the missing link  PsyPost Source link

Summary: New research has uncovered that neurons store their own glycogen, acting as “backup batteries” to keep the brain functioning during energy stress. Using worms and advanced biosensors, scientists found that neurons can tap into glycogen reserves, especially when oxygen or mitochondrial function is limited. This metabolic flexibility, termed “glycogen-dependent glycolytic plasticity,” shows neurons are more self-sufficient than previously believed. …