Tag Archives: Mitochondrial

Reagents Details for chemicals, antibodies, primers and other reagents used are listed in Supplementary Tables 4 and 5. Amyloid-β oligomers were prepared from HFIP-dried or TFA-precipitated peptides (r-Peptide). TFA-peptides were solubilized into monomers with ice-cold HFIP for 2 h before evaporating overnight in a chemical safety cabinet. Oligomerization-state was evaluated by western blotting, as previously described103. S3QEL2 was sourced from Cayman …

Cell culture, transfection and RNA interference MEFs and HeLa cells were maintained in DMEM-GlutaMAX (Thermo Fisher Scientific) containing 4.5 g l−1 of glucose supplemented with 1 mM sodium pyruvate (Gibco), 100 μM non-essential amino acids (Gibco) and 10% FBS (Biochrom). IMR90 cells were maintained in MEM-GlutaMAX (Thermo Fisher Scientific) and 10% FBS. MEF and HeLa cell lines were maintained at 37 °C under 5% CO2 …

Caltech scientists discovered that many mitochondrial proteins begin entering the organelle while they are still being made, overturning decades of assumptions. Credit: Shutterstock Researchers showed that many mitochondrial proteins enter the organelle during synthesis, guided by folding patterns and structural signals. This discovery revises decades of biochemical models. Mitochondria are organelles most commonly known as the “powerhouses of the cell” …

Competing interests M.J.F. has served as a paid consultant with Minovia Therapeutics (last in 2021) and Imel Therapeutics (last in 2022), and has served as a preclinical stage research collaborator with Minovia Therapeutics (last in 2022) and Imel Therapeutics (last in 2022). M.J.F is also engaged with biopharma companies involved in mitochondrial disease therapeutic preclinical and/or clinical-stage development, including as …

Eight babies have been born free of a disease that can lead to terrible suffering and early death, thanks to pioneering scientists in the UK employing a form of genetic engineering that is banned in some countries, including the US and France. Ten years ago, when the government and regulators were considering whether to allow mitochondrial transfer technology, critics warned of “Frankenstein meddling” …

Facebook Tweet Email Link London AP  —  Eight healthy babies were born in Britain with the help of an experimental technique that uses DNA from three people to help mothers avoid passing devastating rare diseases to their children, researchers reported Wednesday. Most DNA is found in the nucleus of our cells, and it’s that genetic material — some inherited from …