Tag Archives: Mitochondria

Isolation of nuclei from post-mortem brain tissue Batch 1 nuclei (BA10 region, frozen tissue) were isolated according to a protocol adapted from a previous study18, performed entirely at 4 °C or on ice. In brief, tissue was homogenized (700 µl homogenization buffer: 320 mM sucrose, 5 mM CaCl2, 3 mM Mg(CH3COO)2, 10 mM Tris-HCl pH 7.8, 0.1 mM EDTA pH 8.0, 0.1% IGEPAL CA-630, 1 mM β-mercaptoethanol, 0.4 U µl−1 recombinant RNase …

Oxford scientists have found that sleep may be triggered by tiny energy leaks in brain cell mitochondria, suggesting our nightly rest is a vital safety mechanism for the body’s power supply. Credit: Stock A new study reveals that a buildup of metabolism in specialized brain cells is what triggers the need for sleep. Sleep may serve as more than rest …

While chemotherapy remains a cornerstone of lung cancer treatment, it often weakens the immune system it relies on for long-term control. Now, researchers have found a way to turn this weakness into strength — by transplanting healthy mitochondria into the tumor environment. In advanced non-small cell lung cancer (NSCLC), combining mitochondrial transplantation with cisplatin not only enhanced immune cell infiltration …

Mitochondria have primarily been known as the energy-producing components of cells. But scientists are increasingly discovering that these small organelles do much more than just power cells. They are also involved in immune functions such as controlling inflammation, regulating cell death and responding to infections. Research from my colleagues and I revealed that mitochondria play another key role in your …