Tag Archives: Miniature

A miniature CRISPR–Cas10 enzyme confers immunity by inhibitory signalling

A miniature CRISPR–Cas10 enzyme confers immunity by inhibitory signalling

Bacterial strains and growth conditions Standard bacterial cultures were grown in lysogeny broth (LB Lennox) at 37 °C and 250 rpm except where stated otherwise. When necessary, 34 µg ml−1 chloramphenicol (+Ch), +50 µg ml−1 kanamycin sulfate (+K) or +50 µg ml−1 carbenicillin (+C) was supplemented to media and LB agar plates. For cellular assays, all bacterial strains were stored at −80 °C in 25% glycerol (Sigma) when not …

Read More »

Gus Van Sant’s Miniature ‘Dog Day Afternoon’

Gus Van Sant’s Miniature ‘Dog Day Afternoon’

It was “Dog Day Afternoon” in miniature, though with more loony-tunes firepower. On Feb. 8, 1977, Tony Kiritsis, a disgruntled resident of Indianapolis, walked into the offices of the Meridian Mortgage Company and took one of its executives, Dick Hall, hostage. He wired the sawed-off muzzle of a 12-gauge Winchester shotgun to the back of Hall’s head. One end of …

Read More »