Tag Archives: microglia

Human iPS cell lines and culture The human iPS cell lines used for ATAC–seq (Supplementary Table 1) were derived at Rutgers University Cell and DNA Repository (RUCDR). The human iPS cell lines were generated using the Sendai virus method to ensure that they are integration-free and underwent the following quality-control procedures: immunofluorescence staining for pluripotency, mycoplasma contamination test, in-house RNA-seq-based …

Human tissue samples De-identified human specimens were collected from the Autopsy Service in the Department of Pathology at the University of California San Francisco (UCSF) (Supplementary Table 2) with previous patient consent in strict observance of the legal and institutional ethical regulations. Autopsy consents and all protocols for human prenatal brain tissue procurement were approved by the Human Gamete, Embryo and …

Migration of transplanted allogeneic myeloid cells into the brain following systemic hematopoietic stem and progenitor cells transplantation (HCT) holds great promise as a therapeutic modality to correct genetic deficiencies in the brain such as lysosomal storage diseases.1–3 However, the toxic myeloablation required for allogeneic HCT can cause serious, life-threatening side effects limiting its applicability. Moreover, transplanted allogeneic myeloid cells are …