Tag Archives: methylome

Tissue slide preparation Mouse C57 embryo sagittal frozen sections (MF-104-11-C57 and MF-104-13-C57) were purchased from Zyagen. Freshly collected E11 or E13 mouse embryos were snap frozen in optimal cutting temperature (O.C.T.) compounds and sectioned at 7–10 μm thickness. Tissue sections were collected on poly-l-lysine-coated glass slides (Electron Microscopy Sciences, 63478-AS). Juvenile mouse brain tissue (P21) was obtained from the C57BL/6 mice …

Multimodal profiling of human SAT cell types To investigate the cell-type-level epigenomic landscape of human SAT, we profiled DNA methylation, chromatin conformation and gene expression on nuclei isolated from SAT biopsies from Finnish females (Fig. 1 and Supplementary Note 1.1). We used snm3C-seq to simultaneously profile single-cell-level DNA methylation and chromatin conformation of nuclei isolated from five SAT biopsies (see …