Cell culture, transfection and RNA interference MEFs and HeLa cells were maintained in DMEM-GlutaMAX (Thermo Fisher Scientific) containing 4.5 g l−1 of glucose supplemented with 1 mM sodium pyruvate (Gibco), 100 μM non-essential amino acids (Gibco) and 10% FBS (Biochrom). IMR90 cells were maintained in MEM-GlutaMAX (Thermo Fisher Scientific) and 10% FBS. MEF and HeLa cell lines were maintained at 37 °C under 5% CO2 …
Read More »Ribonucleotide incorporation into mitochondrial DNA drives inflammation
