Zebrafish husbandry Stable transgenic zebrafish lines were kept at 28.5 °C in a dedicated aquatics facility with a 14 h on/10 h off light cycle. Casper fish with the following genotype were used for all experiments: mitfa-BRAFV600E;p53−/−;mitfa−/−. Fish were anaesthetized using Tricaine (MS-222; stock concentration of 4 g l−1), diluted until the fish were immobilized. All animal procedures were approved by the Memorial Sloan Kettering …
Read More »Mechanical confinement governs phenotypic plasticity in melanoma
