Strain culture and DNA extraction We used a collection of S. cerevisiae isolates that were previously sequenced using short-read sequencing19. For each isolate, we obtained single colonies from frozen stock on solid YPD (1% yeast extract, 2% peptone, and 2% glucose) and cultured one colony per strain in 25 ml of liquid YPD at 30 °C under shaking (120 rpm). After the culture …
Read More »From genotype to phenotype with 1,086 near telomere-to-telomere yeast genomes
