Tag Archives: gastrulation

Drosophila stocks and genetic crossings To generate fluorescent cephalic furrow mutants, we performed genetic crosses using the loss-of-function alleles btdXA (FBal0030657), eve3 (FBal0003885), prd4 (FBal0013967), slpΔ34B (FBal0035631) and stg2 (FBal0247234); the membrane fluorescent marker Gap43-mCherry (FBal0258719; a gift from K. Skouloudaki); and the green fluorescent balancers FM7c, Kr-GFP (FBst0005193), CyO, twi-GFP (gift from A. Jain) and TM3, Kr-GFP (FBst0005195). We …

Experimental animals and embryo collection D. melanogaster embryos were collected on apple juice agar plates with yeast paste at 22 °C or at the temperatures indicated below. Laboratory cultures of M. abdita (Sander strain) were maintained as described52. M. abdita embryos were collected on apple juice agar plates with fish food paste at 25 °C. The laboratory cultures of C. riparius (Bergstrom …