Protein expression and purification In Figs. 1–4 and 5d, we measured EYFP with the mutations S2insL/S65G/V68L/S72A/T203Y/H231L of avGFP, with an additional 6x His tag on the N-terminus (the full amino acid sequence is given at the end of this section). The protein was expressed using a pET vector constructed using HiFi assembly from Addgene plasmids 78466 and 29653. The resulting vector …
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