Tag Archives: enzyme

Enzyme specificity prediction using cross attention graph neural networks

Enzymes are the molecular machines of life, and a key property that governs their function is substrate specificity—the ability of an enzyme to recognize and selectively act on particular substrates. This specificity originates from the three-dimensional (3D) structure of the enzyme active site and complicated transition state of the reaction1,2. Many enzymes can promiscuously catalyze reactions or act on substrates …

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A miniature CRISPR–Cas10 enzyme confers immunity by inhibitory signalling

A miniature CRISPR–Cas10 enzyme confers immunity by inhibitory signalling

Bacterial strains and growth conditions Standard bacterial cultures were grown in lysogeny broth (LB Lennox) at 37 °C and 250 rpm except where stated otherwise. When necessary, 34 µg ml−1 chloramphenicol (+Ch), +50 µg ml−1 kanamycin sulfate (+K) or +50 µg ml−1 carbenicillin (+C) was supplemented to media and LB agar plates. For cellular assays, all bacterial strains were stored at −80 °C in 25% glycerol (Sigma) when not …

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Enzyme unique to modern humans may have given us an evolutionary edge, research finds

Enzyme unique to modern humans may have given us an evolutionary edge, research finds

Facebook Tweet Email Link Modern humans are evolutionary survivors, thriving generation after generation while our ancient relatives died out. Now, new research into our brain chemistry suggests that an enzyme unique to Homo sapiens may have made us more efficient water seekers than our closest extinct relatives. About 600,000 years ago, modern humans genetically diverged from the lineage that produced …

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