Tag Archives: Engineered

Cell lines The mouse prostate cancer cell line PTEN−/− Kras (PTEN–Kras) derived from 10-week K-ras G12D;PTEN deletion mutant prostate cells was a kind gift from David Mulholland (The Icahn School of Medicine at Mount Sinai)42. The Ras/Myc transformed prostate cancer line, RM9, was a kind gift from Timothy C. Thompson (MD Anderson Cancer Centre) and has been previously described45. ID8, …

IN A NUTSHELL 🧬 Scientists have engineered Salmonella bacteria to trigger immune responses against tumors. 🐭 Tests on mice showed promising reduction in tumor growth. ⚠️ There are potential risks like unintended infections and interactions with the microbiome. 🔬 Research could pave the way for living drug therapies in cancer treatment. In a groundbreaking development, scientists from the Yong Loo …

Over the past 40 years, Dr. Timothy Lepore has been the emergency room head, sole surgeon and medical examiner on Nantucket, a small island off Cape Cod, Massachusetts. Today he runs the only private practice, where he treats dozens of patients with Lyme disease each year. About 15% of residents on the island have been afflicted with the disease, which …

Mammalian cell culture All mammalian cell cultures were maintained in a 37 °C incubator at 5% CO2. HEK293T human embryonic kidney (Thermo Fisher), A549 human lung cancer (a gift from S. Garg) and HeLa human cervical cancer (a gift from M. Stewart) cells were maintained in Dulbecco’s modified eagle’s medium with high glucose, sodium pyruvate and GlutaMAX (DMEM; 10569, Thermo Fisher) …

And, well, it worked remarkably well. The plants carrying all the genes for the McG cycle weighed two to three times as much as control plants that only had some of the genes. They had more leaves, the leaves themselves were larger, and the plants produced more seeds. In a variety of growing conditions, the plants with an intact McG cycle …

Strains, culture conditions and chemicals Escherichia coli strain DH5α was used for plasmid construction. E. coli was grown at 37 °C and 300 r.p.m. in lysogeny broth (LB) liquid medium and at 37 °C on plates of LB solid medium supplemented with 20 g l–1 agar. Ampicillin was supplemented at a concentration of 100 mg l–1 for plasmid selection. The Y. lipolytica W29 strain (MATa, Y-63746 ARS Culture Collection, …

IN A NUTSHELL 🌾 The Moon-Rice project is developing “super-dwarf” rice to support long-duration space missions and extreme Earth environments. 🛰️ Led by the Italian Space Agency, the project involves collaboration between three Italian universities specializing in rice genetics, crop physiology, and space crop production. 🔬 The research focuses on using CRISPR-Cas technology to create compact, productive rice varieties that …