Cell strains The E. coli K-12 MG1655 strain was grown in Luria–Bertani medium at 37 °C with shaking at 200 rpm until reaching an OD600 of approximately 0.6. For heat shock treatment, cells were incubated in a water bath at 44 °C for 10 min. For transcription inhibition, cells were treated with 25 or 750 µg ml−1 Rif for 40 min at 37 °C and 200 rpm. To induce …
Read More »
Facebook Tweet Email Link After a three-year legal battle, the Uvalde Consolidated Independent School District has released records related to the 2022 mass shooting that killed 19 children and two adults in Uvalde, Texas. Local officials came under widespread criticism for the delayed response to the massacre at Robb Elementary School on May 24, 2022. None of the 376 law …
Read More »
article 28-year-old Andrew James McGann (Source: Washington County, Arkansas jail) DALLAS – The suspect accused of killing a married couple who were hiking with their children at Devil’s Den State Park in Arkansas was a teacher in Flower Mound. Devil’s Den Attack 28-year-old Andrew James McGann (Source: Washington County, Arkansas jail) What’s new: Arkansas State Police said 28-year-old Andrew James …
Read More »