Tag Archives: coli

New research suggests that nearly 1 in 5 urinary tract infections (UTIs) in Southern California may be caused by strains of Escherichia coli that originated in food-producing animals. For the study, a team led by scientists at George Washington University and Kaiser Permanente Southern California (KPSC) conducted molecular analysis of more than 5,700 extraintestinal pathogenic E coli (ExPEC) isolates collected …

Cell strains The E. coli K-12 MG1655 strain was grown in Luria–Bertani medium at 37 °C with shaking at 200 rpm until reaching an OD600 of approximately 0.6. For heat shock treatment, cells were incubated in a water bath at 44 °C for 10 min. For transcription inhibition, cells were treated with 25 or 750 µg ml−1 Rif for 40 min at 37 °C and 200 rpm. To induce …